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Open Access Short report

Appendiceal spirochaetosis in children

Laurens J Westerman1, Marguerite EI Schipper2, Herbert V Stel3, Marc JM Bonten1 and Johannes G Kusters1*

Author Affiliations

1 Department of Medical Microbiology, University Medical Centre Utrecht, PO-box 85500, 3508 GA, Utrecht, The Netherlands

2 Department of Pathology, University Medical Centre Utrecht, PO-box 85500, 3508 GA, Utrecht, The Netherlands

3 Department of Pathology, Tergooiziekenhuizen, PO-box 10016, 1201 DA, Hilversum, The Netherlands

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Gut Pathogens 2013, 5:40  doi:10.1186/1757-4749-5-40

Published: 13 December 2013

Abstract

Background

Acute appendicitis is a surgical emergency in which the appendix is surgically removed to prevent peritonitis due to perforation of the appendix. Depending on age and gender, up to 17% of removed appendices do not show the histopathological changes pathognomonic for acute appendicitis and are called ‘pseudo-appendicitis’. Intestinal spirochaetes have been reported in up to 12.3% of these non-inflamed appendices obtained from adults. Although children carry the highest risk for acute appendicitis, not much is known on the prevalence of intestinal spirochaetes in children. The aim of this study was to determine whether there is an association between pseudo-appendicitis and appendiceal spirochaetosis in children.

Methods

Archival appendix specimens from paediatric patients (less than 18 years old) were obtained from two Dutch hospitals (acute appendicitis, n = 63; pseudo-appendicitis, n = 55; control appendices, n = 33) and microscopically analysed by H&E staining and spirochaete-specific immunohistochemistry and Brachyspira species specific real-time PCR.

Results

Five out of 142 appendices were found to be positive, all in male patients: one in the acute appendicitis group, two in the pseudo-appendicitis group and two in the control group.

Conclusion

The results obtained do not provide evidence for a role of Brachyspira species infection in the aetiology of acute appendicitis in children.

Keywords:
Appendicitis; Spirochaetes; Brachyspira species; Human intestinal spirochaetosis; Appendiceal spirochaetosis; Real-time PCR