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Open Access Research

Use of REP- and ERIC-PCR to reveal genetic heterogeneity of Vibrio cholerae from edible ice in Jakarta, Indonesia

Diana E Waturangi1*, Ignasius Joanito1, Yogiara Yogi1 and Sabu Thomas2

Author Affiliations

1 Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jalan Jenderal Sudirman 51, Jakarta 12930, Indonesia

2 Cholera and Environmental Biology Lab, Rajiv Gandhi Centre for Biotechnology (Dept. of Biotechnology, Govt. of India), Trivandrum-695 014, Kerala, India

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Gut Pathogens 2012, 4:2  doi:10.1186/1757-4749-4-2

Published: 15 March 2012

Abstract

Background

Vibrio cholerae is the causative organism of waterborne disease, cholera. V. cholerae has caused many epidemics and pandemics of cholera for many years. In this study, V. cholerae recovered from edible ice were investigated for their genetic diversity using Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR and Repetitive Extragenic Palindromic (REP) PCR. Isolation was done using selective medium and the presumptive isolates were confirmed through biochemical and serological assays.

Results

Seventy-five isolates of V. cholerae were recovered from ice samples collected from different locations of Jakarta. Specifically, 19 of them were identified as O1 serotype, 16 were Ogawa, 3 isolates were Inaba and the remaining isolates were non-O1. The fingerprinting profiles of V.cholerae isolated from ice samples were very diverse.

Conclusion

This result showed that the ERIC sequence is more informative and discriminative than REP sequence for analysis of V. cholerae diversity.

Keywords:
Vibrio cholerae; ERIC-PCR; REP-PCR; Edible ice