Figure 1.

Study Overview. This figure shows an overview of the key events associated with the Clostridium difficile induced colitis model that was used for this study. Female C57BL/6 mice were dosed during study days 0 through 15 with 2 × 10⁹ CFU of BC30, or vehicle (0.9% saline). On study days 5 through 8, mice were given an antibiotic coktail in the drinking water as described in the Methods section. On day 10, clindamycin was administered i.p. at a dose of 10 mg/kg. On day 11, mice received either Clostridium difficile (designated as C diff) [1 × 10⁴ CFU of VPI 10463], or vehicle, by oro-gastric gavage. Body weight and stool consistency data were collected daily on study days 11 through 16. On study day 16, mice were euthanized and the colons were removed for measuring morphometric, biochemical and histological indices of colitis.