Inhibition of CD-9689 to Caco-2 monolayer by cell free supernatants from different lactobacilli. CD-9689 cells were suspended in 1:1 volume/volume RCM- lactobacillus conditioning MRS at a MOI of 100: 1 and incubated at 37°C in 7% CO2 for 2 h. After incubation, Caco-2 cells were washed, stained with Giemsa and counted. The above formula was used to calculate the percent inhibition of adhesion CD-9689 to Caco-2 monolayer. Values are presented as Mean ± Standard error of mean (SEM) of three experiments done in duplicate. ** P < 0.01.
Banerjee et al. Gut Pathogens 2009 1:8 doi:10.1186/1757-4749-1-8