Figure 1.

Role of LDB B-30892 on CD-9689 mediatedcytotoxicity. Confluent Caco-2 cultures were incubated 24 h in DMEM containing 20% FBS. Cell free supernatants (CFS or CFT) from different treatments were added to the cell monolayer. (A) Caco-2 control cells (200× original magnification); (B) Normal-appearance after 24 h of incubation with fresh RCM diluted 1:4 into DMEM containing 20% FBS; (C) Appearance of Caco-2 in LDB B-30892 CFS, diluted 1:4 into DMEM, 20% FBS. (D) Caco-2 cultures incubated 24 h in cell-free RCM after growing CD-9689 24 h in it. Note the cytopathic effect of the CD-9689 cytotoxin. (E) Caco-2 cultures incubated 24 h in cell-free RCM after growing CD-9689 and LDB B-30892 24 hours together in it. (F) Caco-2 cultures incubated 24 h in cell-free RCM after growing CD-9689 for 24 h.